Cast:
- Batman - Buzz Lightyear (Toy Story)
- Robin - Lincoln Loud (The Loud House)
- Alfred Pennyworth - Gru (Despicable Me)
- Barbara Gordon/Batgirl - Jessie (Toy Story 2)
- Mayor McCaskill - Honey Lemon (Big Hero 6)
- The Joker - Emperor Zurg (Toy Story)
- Harley Quinn - Nyra (Legend of the Guardians: The Owls of Ga'Hoole)
- Harvey Dent/Two Face - Tank Evans (Surf's Up)
- The Riddler - Nigel (Rio)
- The Scarecrow - Lord Hater (Wander Over Yonder)
- Catwoman - Chef (Trolls)
- Clayface - Linnux (Rock Dog)
- Poison Ivy - Lola (Shark Tale)
- Killer Croc - Dave (Penguins of Madagascar)
- Orca - Kron (Dinosaur)
- Comissioner James Gordon - King Fergus (Brave)
- Superman - Bob Parr/Mr. Incredible (The Incredibles)
- Phylis - Queen Elinor (Brave)
- Green Lantern - Lucius Best/Frozone (The Incredibles)
- The Flash - Lightning McQueen (Cars)
- Lord Voldemort - Hal Stewart (Megamind)
- King Kong - Captain Gutt (Ice Age: Continental Drift)
- Sauron - Tamatoa (Moana)
- Mr. Freeze - Professor Poopypants (Captain Underpants: The First Epic Movie)
- Bane - Victor Quartermaine (Wallace and Gromit in the Curse of the Were-Rabbit)
Other cast
- Medusa - Scarlet Overkill (Minions)
- Swamp Creature -
- Wicked Witch of the West -
- Agent Smith - Francis E. Francis (The Boss Baby)
- Anchorman Phil -
- Reporter Pippa - Shandra Jimenez (Gravity Falls)
- Chief O'Hara -
- G.C.P.D. Officer - Ronin (Epic)
- Captain Dale - Chance Sureshow (Regular Show)
- Pilot Bill - Colonel Rawls (Regular Show)
- Mark Jonathan Davis - Lynn Loud Sr. (The Loud House)
- Richard Cheese - Ruberiot (Star vs. the Forces of Evil)
- Reporter #1 - Henry Gardner (Storks)
- Reporter #2 - Del Hanlon (Regular Show)
- Reporter #3 - Frank Smith (Regular Show)
Buzz Lightyear as Batman
Lincoln Loud as Robin
Gru as Alfred Pennyworth
Metal Beak as The Joker